The Tsuchigumo village is the village of the Tsuchigumo clan. It is led by the Tsuchigumo chief. This village is composed of the members of the Tsuchigumo clan who refused to follow En no Gyōja to Mount Katsuragi, and consequently denied the Tsuchigumo clan kinjutsu. They were made into pawns by the Magaki Group, who sought the kinjutsu.
When Team Yamato brings Hotaru back to the Tsuchigumo village, the people of the village weren't pleased to see her again. The chief approaches them seemingly happy to see Hotaru's return to the village. As Team Yamato leaves, he takes Hotaru to a room to rest, only to lock her in there with the Magaki Group who is after the Tsuchigumo clan kinjutsu. After Hotaru escapes the ninja bandits and they follow her, Naruto returns to the village and asks the chief what happened. He tells Naruto that the village has yet to begin their search for Hotaru, so Naruto and his team go search for her.
They later appear as a zombie-like horde, used by the Magaki group as cannon-fodder against Sakura, Sai, Yamato, Naruto and Utakata. When the two jinchūriki elect to go on ahead, Sai, Yamato and Sakura hold both them and distract the Magaki Group.