"The Weakness of Despair!!" (絶望の弱点!!, Zetsubō no Jakuten!!, Viz: The Weak Point of Despair) is chapter 578 of the original Naruto manga.
Having arrived at Kabuto's hideout, Sasuke identifies Kabuto. Kabuto attempts to manipulate him into attacking Itachi, but Sasuke explains his goal was to talk with his brother and that Kabuto, who has now taken Orochimaru's mantle, is his enemy. Meanwhile Tsunade reveals her new technique's power, which allows instant regeneration without the usage of hand seals, and throws the sword that impales her back at Madara. While he absorbs it into his Susanoo, Ōnoki tries to blast Madara with Dust Release from behind, but Madara simply absorbs it, claiming he could have done it earlier but instead took it as a chance to demoralise them with Hashirama's visage. Tsunade questions his confidence, stating the Kage forced him into using a wood clone. Instead of rebuffing her, Madara admits five versus one is a good match-up number and creates twenty-five wood clones, five for each Kage. Sasuke attempts to kill Kabuto, but Itachi stops him, explaining the need to discover the counter to the Impure World Reincarnation beforehand. Sasuke agrees to cooperate on the terms that Itachi will answer his questions once they're done, and Itachi accepts, as both brothers and Kabuto ready themselves for battle.