The Two Great Sages…!! (二大仙人…!!, Ni Daisennin…!!) is chapter 375 of the Naruto manga.
Jiraiya tells Gamaken to buy him time while he prepares for Sage Mode. Pain hides within his chameleon and summons other animals to fight Gamaken. While Gameken defends against them, Jiraiya points out that Pain, having the Rinnegan, can use almost any jutsu, yet he has so far used only animal summons. Jiraiya sends Gamaken away once he is ready, blocking a summon by himself and reentering the battle with two new toads.