He and his partner, Yūdachi were captured by Jiraiya. When they refused to tell Jiraiya any information on Pain, even after tickle torture on his partner, he transformed Yūdachi into a frog, which frightened Ryūsui into divulging information about Pain. Jiraiya then tries to control Ryūsui in an attempt to find Pain, but is found by Konan. Ryūsui then follows Konan's orders to leave and is able to escape.
The anime expands on his situation, seeing as he is captured by Jiraiya who learned about him from his wife, Tezuna.