Izanagi and Izanami (イザナギとイザナミ, Izanagi to Izanami) is episode 338 of the Naruto: Shippūden anime.
With Izanami activated, and Kabuto trapped within its infinite loop, Sasuke uses this time to question his brother about the technique. Itachi tells Sasuke about the history behind Izanami's creation as well as the Uchiha clan's use of the technique. Itachi ultimately reveals to Sasuke that Izanami was created with the sole purpose of stopping the users of the technique's counterpart: Izanagi so that they would accept their fate — which is also the way to escape the infinite loop. Angered by the fact that Itachi would cast a technique that has an escape route, Sasuke questions his motives to which Itachi says that he wished to give Kabuto an opportunity to accept his fate. Sasuke, however, claims that Itachi is perfect and rather different from Kabuto but the elder Uchiha refuses this claim. Elsewhere, somewhere in a forest seemingly some distance away, a grown-up Urushi tells his comrade that he hoped by the time he gets home, Kabuto will be there. Back at the cave, Itachi prepares to put an end to the Impure World Reincarnation technique.
In the preview of this episode, the flashback of the Uchiha inter-clan rivalry was shown in colour. However, this episode shows the flashback in black and white in its airing.
Itachi uses Tsukuyomi on Kabuto with his "right Mangekyō", even though his Tsukuyomi eye is his "left Mangekyō", which had lost its sight earlier in battle due to the use of Izanami.
This is the only episode to show a female Uchiha clan member using the Sharingan.
When Itachi talked with Sasuke before he decided to release the Impure World Reincarnation, Itachi's left sclerae mistakenly appeared normal in one scene instead of black sclerae.