"In Attendance, the Six Paths of Pain" (ペイン六道、見参, Pein Rikudō, Kenzan) is episode 132 of the Naruto: Shippūden anime.
Jiraiya blinds one of the new Pains with a kick to the face, and Fukasaku suspects that all three Pain's share the same eyes and vision, while Jiraiya realises that each of the bodies has only one specific purpose. Jiraiya stalls for time while Fukasaku and Shima prepare a powerful genjutsu to defeat the bodies and are successful in doing so. As Jiraiya leaves, another Pain ambushes Jiraiya, destroying his left arm. Jiraiya looks up to see Six Pains, including the three killed before, leaving him horrified and wondering what Pain really is.