Jiraiya decides to use Sage Mode in his battle against Pain but needs time to prepare. Clasping his hands together and unable to open them, lest he interrupt his preparation, Jiraiya relies on Gamaken to protect and defend him. Pain uses camouflage to turn himself invisible, while Jiraiya erects a spherical detection barrier to locate him. Pain summons a Giant Multi-Headed Dog. Gamaken is unable to defeat the dogs due to the fact that they are able to multiply when hit; the dogs eventually corner Gamaken and bite into him, wounding him severely. Jiraiya uses his Summoning: Crushing Toad Stomach to reverse summon himself, Gamaken and the dogs into the stomach of a toad, where the toad walls begin to absorb the dogs, which dispel themselves.
Pain states that the battle is over as Jiraiya had escaped, only for Jiraiya and Gamaken to have located the camouflaged Pain. Gamaken is able to strike at Pain's Giant Snake-Tailed Chameleon, although Pain summons a Giant Drill-Beaked Bird to dive bomb itself towards Jiraiya and Gamaken as retribution. Jiraiya begins to run away, his hands still clasped, while being chased by the bird. However, after tricking the bird into coming face-to-face with him, Jiraiya incinerates the bird with his Fire Release: Flame Bullet. After utilising shadow clones to aid him against Pain's Giant Ox and Giant Rhino, Jiraiya is finally able to call uponFukasaku and Shima, the Two Great Sage Toads of Mount Myōboku. He tells them that he has summoned them due to the fact that his enemy possesses the Rinnegan, the mythical dojutsu once wielded by the Sage of Six Paths. To make matters worse, he believes Pain is the Child of the Prophecy, one who the Great Toad Sage prophecised would bring either peace or destruction upon the world. As Pain fits into the latter category, Jiraiya and the toads realise that it is Jiraiya's duty to kill Pain, who summons his other Paths.