"A Clan Possessed by Evil" (悪に憑かれた一族, Aku ni Tsukareta Ichizoku) is chapter 619 of the original Naruto manga.
As the newly reincarnated Hokage adjust to their circumstance, Suigetsu marvels at being in the presence of Hashirama. When the situation of how their souls were released and then they were reincarnated is explained by Hiruzen and Minato, Hashirama bluntly asks who the latter was. Announcing himself via his title of Fourth Hokage, Hashirama is pleased that the village had seemingly enjoyed such stability but was later shocked and bit apprehensive to hear that his granddaughter had inherited the title of Fifth Hokage. The banter between the Hokage is soon cut short after Orochimaru allayed their outrage that he had reincarnated them to destroy Konoha and instead presented Sasuke to them. From this point, Sasuke first questioned the Third about the Uchiha clan massacre and Hiruzen sombrely confirmed all that the young man had been told, noting that this was something Itachi decided to shoulder himself. This led the conversation to segue into a conversation about the Uchiha with Tobirama and his decision, as well as motives behind giving the clan the Konoha Military Police Force as a sign of trust. Here it was revealed that the Uchiha was a clan possessed by evil, but above even the Senju, they valued love and friendship above all else, but as a result of this, its loss had an even greater-than-normal effect on them. Upon hearing all this, the young Uchiha turns towards the once-leader of the Senju clan, posing two questions to him: what the village meant, and what was the purpose of shinobi.
Going from timeline of events given in the databooks, Hashirama would not have been alive when Tsunade was born.